Journal: Nature Communications

Article Title: Jouvence a small nucleolar RNA required in the gut extends lifespan in Drosophila

doi: 10.1038/s41467-020-14784-1

Figure Lengend Snippet: Longevity test results (survival curve − decreasing cumulative) of the targeted expression of jou specifically in the enterocytes in Wild-Type genetic background compared to their controls. Myo1A-Gal4>UAS-jou 8M ( a ) and Mex-Gal4>UAS-jou 8M ( b ) overexpression in enterocytes is sufficient to increase lifespan. In each longevity panel, Control-1 = Gal4-driver/+, meaning the Gal4 driver without the UAS-construct, while Control-2 = UAS-construct/+, meaning the UAS-jou 8M , or sno-2, or sno-3, without the Gal4 driver (Notice: same nomenclature for all other overexpression longevity Figures). c Overexpression of jou only in adulthood is sufficient to increase lifespan (Mex-GS>UAS-jou 8M flies fed with RU486 only in adulthood). d , e Overexpression of the sno-2 ( d ) or sno-3 ( e ) only in adulthood do not have any effect on lifespan (Mex-GS>UAS-sno-2 or sno-3 flies fed with RU486 only in adulthood) (*** = p < 0,001) (for number of flies, age in days at % mortality, and detailed statistics, see Supplementary Information, Table ). p -value calculated by log-rank test. f RT-qPCR (Taqman) results of the targeted overexpression of jou specifically in the enterocytes compared to their controls Myo1A-Gal4, and Mex-Gal4, respectively. Two assay-repetitions were done ( n = 2) for each genotype. g RT-qPCR (Taqman) results of the RU486 induced targeted overexpression of jou specifically in the enterocytes only in adulthood in Mex-GS ( n = 4), compared to the non-induced controls flies ( n = 7). For f , g the snoRNA- jouvence is normalized to the level of rp49. For each condition, 30 guts were dissected for the total RNA extraction. Statistics: compared to control Myo/CS, or Mex/CS, or to the non-induced Mex-GS: ( p -values) (* p < 0,05; ** p < 0,005; *** p < 0,0005). Errors bars represent the mean ± S.E.M. ( p -value were calculated using the one-way ANOVA followed by a TUKEY test). For all panels, Source data are provided as a Source Data file.

Article Snippet: Mouse and human total RNA were commercially purchased at Amsbio: Mouse Brain, whole Total RNA: MR-201, Mouse Ovary Total RNA: MR-406, Mouse Kidney Total RNA: MR-901, Human Brain Total RNA: HR-201, Human Ovary Total RNA: HR-406, Human Kidney Total RNA: HR-901.

Techniques: Expressing, Over Expression, Construct, Quantitative RT-PCR, RNA Extraction

Journal: Nature Communications

Article Title: Jouvence a small nucleolar RNA required in the gut extends lifespan in Drosophila

doi: 10.1038/s41467-020-14784-1

Figure Lengend Snippet: a Number of enterocytes (ECs) in young (7 day-old: light and dark blue) and aged (40-day-old: light and dark red) flies (light = in WT and dark = in deletion). As expected, the number of ECs is increased (hyperplasia) in aged WT flies compared to young (7 day-old) flies. However, this number is more increased in jou -deleted flies (dark blue and dark red compared to light blue and light red). Remarkably, the number of ECs is not increased in old genomic-rescued flies (Del;rescue-1), as well as in targeted expression of jou in the gut (Del,Myo1A-Gal4>UAS-jou 1M and Del,Mex-Gal4>UAS-jou 1M ). Finally, the overexpression of jou in WT genetic background prevents the hyperplasia (Myo1A-Gal4>UAS-jou 1M and Mex-Gal4>UAS-jou 1M ). b The number of Dl + cells (Dl-Gal4>UAS-GFP-nls) in WT control and jou -deleted flies. c The number of anti-PH3 cells. d The number of EdU labelled cells, both in young (blue) and old flies (red). Co-young: n = 22, Del-young: n = 24, Co-old: n = 31, Del-old: n = 26, Del+rescue-1-old: n = 8, Mex-GS>jou, no RU: n = 8, with RU: n = 7). e Relative Intensity of DAPI-fluorescence used to evaluate the endoreplication. a – e The number at the bottom of each graph represents the number of animals assayed ( n ). f RT-qPCR (SybrGreen) performed on the total RNA extracted from the gut on the cyclin-E gene, used to evaluate/quantify the endoreplication. The expression of the cyclin-E is importantly reduced in jou -deleted flies ( n = 4 for each genotype). Statistics: * p < 0,05; ** p < 0,005; *** p < 0,0005. Errors bars represent the mean ± S.E.M. ( p -value were calculated using the one-way ANOVA followed by a TUKEY test). For all panels, Source data are provided as a Source Data file.

Article Snippet: Mouse and human total RNA were commercially purchased at Amsbio: Mouse Brain, whole Total RNA: MR-201, Mouse Ovary Total RNA: MR-406, Mouse Kidney Total RNA: MR-901, Human Brain Total RNA: HR-201, Human Ovary Total RNA: HR-406, Human Kidney Total RNA: HR-901.

Techniques: Expressing, Over Expression, Fluorescence, Quantitative RT-PCR

Journal: Nature Communications

Article Title: Jouvence a small nucleolar RNA required in the gut extends lifespan in Drosophila

doi: 10.1038/s41467-020-14784-1

Figure Lengend Snippet: a Transcriptomic analysis (RNA-Seq) performed on total-RNA from the gut reveals that 314 genes are upregulated, while 319 are downregulated, in deletion compared to Control-CS (see Supplementary Data and for the complete list of genes). b Clusters analysis (Heat map) of differentially expressed genes. c , d Statistics of pathway enrichment of the deregulated genes according to the KEGG analysis (see Supplementary Data and for the full list of the KEGG analysis). In brief, for the upregulated genes ( c ), the metabolic pathways are the main deregulated pathways, while for the downregulated genes ( d ), the glutathione metabolism is the main deregulated pathway (see the Supplementary Data and for the Gene Ontology Analysis).

Article Snippet: Mouse and human total RNA were commercially purchased at Amsbio: Mouse Brain, whole Total RNA: MR-201, Mouse Ovary Total RNA: MR-406, Mouse Kidney Total RNA: MR-901, Human Brain Total RNA: HR-201, Human Ovary Total RNA: HR-406, Human Kidney Total RNA: HR-901.

Techniques: RNA Sequencing Assay

Journal: Nature Communications

Article Title: Jouvence a small nucleolar RNA required in the gut extends lifespan in Drosophila

doi: 10.1038/s41467-020-14784-1

Figure Lengend Snippet: RT-qPCR (SybGreen) results of the quantification of the GstE5, Gba1a, LysB, ninaD, CG6296, and Cyp4p2 genes in Control (CS), Del (deletion F4), rescue-1, Del+rescue-1, and Del,Myo1A>UAS-jou 8M in deletion genetic background. In deletion, the mRNA level is increased for GstE5, Gba1a, LysB, and ninaD genes, while in contrary it is decreased for CG6296 and Cyp4p2. For the genes GstE5 ( a ), Gba1a ( b ), and LysB ( c ), the mRNA level is restored in genomic-rescued transgenic flies Del+rescue-1 (even it is more than restored in LysB), while inversely for ninaD ( d ), the mRNA level is more increased than in deletion. The targeted expression of jou specifically in enterocytes (Del,Myo1A>UAS-jou 8M ) in deletion is sufficient to restore the level of mRNA for GstE5, Gba1a and LysB, but again here, not for ninaD. For the downregulated genes: CG6296 ( e ) and Cyp4p2 ( f ), none of the transgenic construction (Del+rescue-1 and Del,Myo1A>UAS-jou 8M ) rescues the level of mRNA (although some partial rescued is detectable with Del,Myo1A>UAS-jou 8M for CG6296). g , h Longevity test results (survival curve—decreasing cumulative) of the targeted expression of UAS-ninaD-RNAi ( g ) and UAS-cDNA-CG6296) ( h ) specifically in the enterocytes in deletion genetic background compared to their controls. Restoring the level of ninaD (Del,Mex-GS>UAS-ninaD-RNAi) ( g ) only in adulthood (starting feeding flies with RU486 just after the flies have hatched) nicely increases lifespan compared to the non-RU486 fed control sibling flies). Similarly, restoring the mRNA level of CG6296 gene (Del,Mex-GS>UAS-cDNA-CG6296) ( h ) and so also only in adulthood, importantly increases lifespan compared to their control sibling flies. (*** = p < 0,001) (for number of flies, age in days at % mortality, and detailed statistics, see Supplementary Information, Table ). RT-qPCR performed from the dissected guts of the RU486-induced only in adulthood (Del,Mex-GS>ninaD-RNAi or cDNA-CG6296), compared to the non-induced controls flies, confirm that the mRNA levels decreased (restored) for ninaD (inset g ), and increased (restored) for CG6296 (inset h ). For each panel, the level of the analysed gene has been normalized to the level of rp49. 30 guts were dissected for the total RNA extraction. The number at the bottom of the box (inside or just above) represents the number of samples ( n ). Statistics: RU486-induced compared to control non-induced (*** p < 0,001). Errors bars represent the mean ± S.E.M. ( p -value were calculated using the one-way ANOVA followed by a TUKEY test). For all panels, Source data are provided as a Source Data file.

Article Snippet: Mouse and human total RNA were commercially purchased at Amsbio: Mouse Brain, whole Total RNA: MR-201, Mouse Ovary Total RNA: MR-406, Mouse Kidney Total RNA: MR-901, Human Brain Total RNA: HR-201, Human Ovary Total RNA: HR-406, Human Kidney Total RNA: HR-901.

Techniques: Quantitative RT-PCR, Transgenic Assay, Expressing, RNA Extraction

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Isolated Langerhans cell histiocytosis of the stomach: a case report and literature review

doi:

Figure Lengend Snippet: Immunohistochemical staining for biomarkers in histiocytoid cells. A. Strongly positive staining for S-100 (IHC × 100); B. Strongly positive staining for CD1a (IHC × 100). C. Strongly positive staining for langerin (CD207) (IHC × 100); D. Negative staining for CD21 (IHC × 100); E. Negative staining for BRAF V600E (IHC × 100).

Article Snippet: We used the the following primary antibodies: Vimentin (clone V9, OriGene), S100 (clone poly, OriGene), CD1a (clone O10, OriGene), langerin (clone343828, OriGene), Pan-cytokeratin (clone AE1, OriGene), CD68 (clone PG-M1, OriGene), CD163 (clone 10D6, OriGene), CD21 (clone 2G9, OriGene), CD35 (clone E11, OriGene), CD117 (clone poly, OriGene), CD34 (clone QBEnd-10, OriGene), DOG-1 (clone SP31, OriGene), cyclinD1 (clone DCS-6, OriGene), MLH1 (clone ES05, OriGene), MSH2 (clone 25D12, OriGene), MSH6 (clone EP49, OriGene), PMS2 (clone EP51, OriGene), HP, P53 (clone DO7, OriGene), Ki-67 (clone UMAB107, Ori-Gene).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Negative Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Isolated Langerhans cell histiocytosis of the stomach: a case report and literature review

doi:

Figure Lengend Snippet: Reported cases of adult LCH

Article Snippet: We used the the following primary antibodies: Vimentin (clone V9, OriGene), S100 (clone poly, OriGene), CD1a (clone O10, OriGene), langerin (clone343828, OriGene), Pan-cytokeratin (clone AE1, OriGene), CD68 (clone PG-M1, OriGene), CD163 (clone 10D6, OriGene), CD21 (clone 2G9, OriGene), CD35 (clone E11, OriGene), CD117 (clone poly, OriGene), CD34 (clone QBEnd-10, OriGene), DOG-1 (clone SP31, OriGene), cyclinD1 (clone DCS-6, OriGene), MLH1 (clone ES05, OriGene), MSH2 (clone 25D12, OriGene), MSH6 (clone EP49, OriGene), PMS2 (clone EP51, OriGene), HP, P53 (clone DO7, OriGene), Ki-67 (clone UMAB107, Ori-Gene).

Techniques: Mutagenesis

Journal: Journal of thrombosis and thrombolysis

Article Title: Metal ion chelation enhances tissue plasminogen activator (tPA)-induced thrombolysis: an in vitro and in vivo study.

doi: 10.1007/s11239-021-02600-6

Figure Lengend Snippet: Fig. 5 Photothrombosis and thrombolysis in vivo in the mouse femo- ral artery. A The femoral artery (shown by an arrow) of CD1 mouse. Scale bar: 1 cm. B(1–3) Photothrombotic occlusion of the femoral artery. B-1 Clot indicated by an arrow occluded the femoral artery downstream (outlined by dash lines) of the thrombus formation. The artery occlusion created a blood-free field. B-2. Reperfusion of the femoral artery by the treatment of tPA. B-3 Darker colored clot in a non-reperfused femoral artery. The image (B-3) was taken 30 min after establishing photothrombotic clot. Scale bar for B: 1 mm. C Change of color intensity of clot (thrombus) in non-reperfused femo- ral artery over time. Images of the thrombus were taken every 5 min and was analyzed by ImagePro software. Change of thrombus color intensity was expressed as percentage increase and calculated accord- ing to the formula (F -F0)/F0· %. Values are mean ± SEM. n = 7–9

Article Snippet: Citrated blood of male CD1 mice was purchased from Innovative Research (Novi, MI) and BioChemed Services (Winchester, VA).

Techniques: In Vivo, Software